Table 1.

Summary of posttranslational modification sites of human sirtuins.

Sirtuin	Target site	Type of modification	Modifier	Source
SIRT1	Ser14	P		Sasaki et al. (2008)
	Ser26	P		Sasaki et al. (2008)
	Ser27	P	JNK	Nasrin et al. (2009), Sasaki et al. (2008)
	Ser47	P	JNK, mTOR	Nasrin et al. (2009), Back et al. (2011), Sasaki et al. (2008)
	Ser172	P		Sasaki et al. (2008)
	Ser173	P		Sasaki et al. (2008)
	Lys233	Me	Set7/9	Liu et al. (2011)
	Lys235	Me	Set7/9	Liu et al. (2011)
	Lys236	Me	Set7/9	Liu et al. (2011)
	Lys238	Me	Set7/9	Liu et al. (2011)
	Cys387	NO	GAPDH	Kornberg et al. (2010)
	Cys390	NO	GAPDH	Kornberg et al. (2010)
	Thr530	P	JNK, CycB/CDK1	Nasrin et al. (2009), Sasaki et al. (2008)
	Ser540	P	CycB/CDK1	Sasaki et al. (2008)
	Ser659	P	CK2	Zschoernig and Mahlknecht (2009)
	Ser661	P	CK2	Zschoernig and Mahlknecht (2009)
	Ser719	P		Sasaki et al. (2008)
	Lys734	Sumo	SENP1	Yang et al. (2007)
	Ser747	P		Sasaki et al. (2008)
SIRT2	Ser368	P	CycB/CDK1, CycE/CDK2, CycA/CDK2, CycD3/CDK4, p35/CDK5	North and Verdin (2007b); Pandithage et al. (2008)
	Ser372	P		Nahhas et al. (2007)
		Ac	p300	Han et al. (2008)
SIRT3	Ser101	P		Olsen et al. (2010)
	Ser103	P		Olsen et al. (2010)
	Ser105	P		Olsen et al. (2010)
	Ser114	P		Olsen et al. (2010)
	Ser117	P		Olsen et al. (2010)
	Ser118	P		Olsen et al. (2010)
SIRT4	Ser255	P		Yu et al. (2007)
	Ser261	P		Yu et al. (2007)
	Ser262	P		Yu et al. (2007)
SIRT6		ADPr	SIRT6	Liszt et al. (2005)
	Tyr294	P		Dephoure et al. (2008)
	Ser303	P		Dephoure et al. (2008)

For an overview of the localization of the different modifications relative to other recognizable elements and domains of human sirtuins see Figure 2. Ac, acetylation; ADPr, ADP-ribosylation; Me, methylation; NO, nitrosylation; P, phosphorylation; Sumo, sumoylation; JNK, JUN N-terminal kinase; Cyc, cyclin; CDK, cyclin-dependent kinase. Note that amino acid numbers for SIRT2 refer to the longer isoform 1 (IF1 in Figure 2).