Figure 6. Stromal networks and uNK cells in gd7.5 and gd10.5 B6 implantation sites.

Immunofluorescent reactivity using DBA lectin (red) and gp38/podoplanin (green; A and B) or ER-TR7 (green; C and D) identified decidual stromal regions expressing lymphoid stroma-associated markers and their relationships with uNK cells. The relationships between CD127⁺ cells and the gp38⁺ microenvironment are shown at gd7.5 (E and F) and at gd10.5 (G and H). DAPI (blue) was used to localize cell nuclei. (I) PCR detected transcripts of Aire in adult nonpregnant thymus, DB, and MLAp. H₂O served as the negative control. M, Mesometrial side; AM, antimesometrail side; Em, embryo. Scale bars indicate image magnification. Two or more viable implantation sites from each of multiple [2–5] females were studied.