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. 2012 Mar;91(3):427–435. doi: 10.1189/jlb.0411209

Figure 3. BMMC gp130 receptor expression is induced by IL-10 and dependent on STAT3.

Figure 3.

(A) BMMC rested for 90 min were stimulated with LPS 500 ng/ml or the cytokines shown (each 10 ng/ml) for 3 h and then total RNA isolated and quantitative PCR performed. RNA samples were pooled from three independent experiments and run under the same PCR conditions. *P < 0.01 for IL-10 condition compared with all other conditions. (B) BMMC were cultured for 72 h in conditions including LPS (500 ng/ml), IL-33 (10 ng/ml), or IL-10 (10 ng/ml) and then harvested for flow cytometry to examine gp130 expression on the cell surface. (C) BMMC were cultured with IL-10 (10 ng/ml) for 24 h, with or without the addition of Stattic, an inhibitor of STAT3 activation. Stattic was added to conditions 45 min before the addition of IL-10. (D) BMMC were cultured for 72 h in conditions identical to those in B and then processed for immunoblot using antibodies specific for the gp130 N-terminal. Fresh mouse splenocytes were rested for 2 h and used for comparison. (B–D) Data are representative of at least three experiments with similar results. Shaded histograms (B and C) in flow cytometry data represent isotype control antibody fluorescence.