Figure 5. IL-6 trans-signaling increases BMMC survival under limiting conditions and cytokine secretion.
(A) gp130Y757F BMMC were cultured for 5 days in the conditions indicated, including IL-3 (5 ng/ml), SCF (10 ng/ml), IL-10 (10 ng/ml), or H-IL-6 (100 ng/ml). The gate shown depicts the percentage of events that is Annexin V-negative and Kit-high viable mast cells, and the dot plots represent one of three experiments. (B) Pooled data from the three experiments depicted in A. *P < 0.05; **P < 0.01. (C) gp130Y757F BMMC were cultured overnight in complete media containing IL-3 (5 ng/ml) and IL-10 (10 ng/ml). After this, washed and rested cells were then stimulated in the conditions shown, including IL-33 (10 ng/ml), H-IL-6 (100 ng/ml), or both. Cell-free supernatants were taken after 3 h for IL-13, TNF-α, and MCP-1 cytokine measurement by ELISA, and ELISA data are from two independent experiments, each with four replicates/condition. *P < 0.05; **P < 0.01.