Table 1.

Tidal volume and flow validation study data

Mouse	No. of Breaths	Mean Vt, μl	Mean Difference, μl	SD, μl	Mean Difference 95% CI	Relative Mean Difference, %	CV, %
Vt
1	142	193.2	0.0	7.2	−1.2–1.2	0.0	3.7
2	189	180.4	−6.2	4.8	−6.9–−5.5	−3.5	2.7
3	131	163.8	7.9	4.8	7.1–8.8	4.8	3.0
4	102	195.9	−8.6	4.4	−9.5–−7.7	−4.4	2.3
Total	564	182.6	−1.8	8.9	−2.5–−1.1	−1.0	4.9

Phase	No. of Breaths (5 Per Mouse)	Mean Flow, μl/s	Mean Difference, μl/s	SD, μl/s	Mean Difference	Relative Mean	CV, %
Tidal airflow
Inspiration	20	845.1	−40.5	264.9	−49.5–−31.5	3.7	24.4
Expiration	20	−761.8	−201.7	698.5	−219.4–−184.0	−30.9	107.0

Tidal airflow data set consists of 5 consecutive breaths from 4 individual mice (20 breaths total). Top: mean Vt, mean tracheal tidal volume; mean difference, mean tracheal Vt minus mean whole body plethysmography Vt; SD, SD of mean difference; relative mean difference, mean difference/mean tracheal tidal volume; CV, coefficient of variation = mean difference/mean tracheal Vt. Bottom: mean flow, mean tracheal airflow; mean difference, mean tracheal airflow minus mean whole body plethysmography airflow; relative mean difference, mean difference/mean tracheal airflow; CV, coefficient of variation = mean difference/mean tracheal airflow.