Data on dose optimization are as follows (gene expression was analyzed at 48 hours after intravenous injection)

Quad	X, mean	Y, mean	Events	% Total	% Gated
Dose: 5 μg of pEGFP per rat gene expression: 2.59
1 UL	8.8	10.1	3	0.02	66.01
2 UR	146.4	29.5	26	0.21	2.24
3 LL	2.8	2.3	12113	96.34	31.40
4 LR	73.4	4.7	44	0.35	0.35
Dose: 10 μg of pEGFP pre rat gene expression: 4.92
1 UL	8.8	10.1	3	0.02	67.06
2 UR	146.4	29.5	26	0.21	3.10
3 LL	2.8	2.3	12113	96.34	28.37
4 LR	73.4	4.7	44	0.35	1.82
Dose: 15 μg of pEGFP per rat gene expression: 16.96
1 UL	8.8	10.1	3	0.02	59.12
2 UR	146.4	29.5	26	0.21	10.31
3 LL	2.8	2.3	12113	96.34	23.08
4 LR	73.4	4.7	44	0.35	6.65
Dose: 20 μg of pEGFP per rat gene expression: 26.38
1 UL	8.8	10.1	3	0.02	53.53
2 UR	146.4	29.5	26	0.21	16.40
3 LL	2.8	2.3	12113	96.34	20.37
4 LR	73.4	4.7	44	0.35	9.98
Dose: 25 μg of pEGFP per rat gene expression: 32.96
1 UL	8.8	10.1	3	0.02	46.92
2 UR	146.4	29.5	26	0.21	23.07
3 LL	2.8	2.3	12113	96.34	21.10
4 LR	73.4	4.7	44	0.35	9.89
Dose: 30 μg of pEGFP per rat gene expression: 44.20
1 UL	8.8	10.1	3	0.02	38.35
2 UR	146.4	29.5	26	0.21	31.64
3 LL	2.8	2.3	12113	96.34	17.43
4 LR	73.4	4.7	44	0.35	12.56
Dose: 40 μg of pEGFP per rat gene expression: 39.91
1 UL	8.8	10.1	3	0.02	39.83
2 UR	146.4	29.5	26	0.21	30.17
3 LL	2.8	2.3	12113	96.34	20.28
4 LR	73.4	4.7	44	0.35	9.74
Dose: 50 μg of pEGFP per rat gene expression: 44.38
1 UL	8.8	10.1	3	0.02	39.88
2 UR	146.4	29.5	26	0.21	30.12
3 LL	2.8	2.3	12113	96.34	16.72
4 LR	73.4	4.7	44	0.35	14.26
Dose: 60 μg of pEGFP per rat gene expression: 40.91
1 UL	8.8	10.1	3	0.02	40.15
2 UR	146.4	29.5	26	0.21	32.33
3 LL	2.8	2.3	12113	96.34	19.14
4 LR	73.4	4.7	44	0.35	8.58