Fig. 4.

The expression of G protein subunit candidates Gα_o, Gβ₃, and Gγ₁₃ appears normal in mGluR6-null mice. A and B: Western blots show that the levels of Gα_o (A) and Gβ₃ (B) are the same in WT and mGluR6-null (KO) retinas. Actin is used to control for loading. C: mGluR6 is expressed in the OPL of WT retina, but is absent in mGluR6-null retina. For C–F, fixation and immunostaining of WT and mGluR6-null retinas were performed in parallel, and images were taken with the same settings. D, E, and F: immunostaining for Gα_o (D), Gβ₃ (E), and Gγ₁₃ (F) shows similar expression patterns in WT and mGluR6-null ON bipolar cells. G: average intensity ratios (for immunostaining or Western band intensity) of null to WT retinas for Gα_o, Gβ₃, and Gγ₁₃ in OPL, INL, ON layer in the IPL (IPL-ON), and the sum of these layers + the OFF sublamina (all layers).