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. 2012 Mar;91(3):401–415. doi: 10.1189/jlb.0811394

Figure 1. CD14+CD16+ monocytes increase in numbers in nonadherent culture conditions over 3 days.

Figure 1.

Human monocytes were isolated by CD14-positive selection from PBMCs (two representative individuals are shown, Donor 1 and 2). (A) Freshly isolated and (B) monocytes maturing for 3 days in nonadherent conditions with M-CSF in our culture system were stained with CD14 APC and CD16 PECy7-coupled antibodies. CD14+CD16+ monocytes are present in low numbers in freshly isolated, circulating monocytes. Their numbers significantly increase after 3 days in nonadherent culture conditions. This increase varies among donors.