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. 2012 Mar;26(3):1009–1017. doi: 10.1096/fj.11-194027

Figure 1.

Figure 1.

MRP4 expression and function in the heart. A) Immunodetection of MRP4 in human heart. MRP4 (red) is found on the plasma membrane of human cardiac myocytes in both atria and ventricles, whereas α-actinin (green) is present in the Z line. Scale bars = 10 μm. B) Western blot of membrane fractions from rat atrial myocytes separated on sucrose gradient. Membrane was hybridized to MRP4 antibody (top panel) and to caveolin 3 antibody (bottom panel). C) Representative immunoblot showing a decrease in MRP4 expression. Adult rat ventricular myocytes were infected with either Ad-shScr or Ad-shMRP4 for 3 d (n=5). D) Intracellular (left panel) and extracellular (right panel) cAMP levels in ventricular cardiac myocytes treated with either Ad-shScr or Ad-shMRP4 for 3 d. Cells (200,000/condition) were plated and treated with forskolin (10 μM, 1 h) or the solvent for 1 h. cAMP levels are expressed as picomoles per microgram of protein. Experiments were performed 3 times in triplicate, * P< 0.05; **P < 0.01.