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. 2012 Mar;26(3):1009–1017. doi: 10.1096/fj.11-194027

Figure 2.

Figure 2.

MRP4-deficient mice develop age-dependent cardiac hypertrophy. A) Western blot showing the presence of MRP4 in the heart of WT mice and its absence in Mrp4−/− mice. B) Histology after hematoxylin/eosin labeling and heart weight to tibia length (HW/TL) ratio of hearts from 3- and 9-mo-old Mrp4−/− and WT mice (n=6–10). C) Ventricular myocyte area from 3- and 9-mo-old WT and Mrp4−/− mice, showing cardiac hypertrophy in 9-mo-old Mrp4−/− mouse hearts, compared to age-matched WT (n=3–5). D) Echocardiography parameters showing the left ventricular posterior wall (LVPW) and interventricular septum (IVS) thickness of hearts from 3- and 9-mo-old Mrp4−/− and WT mice (n=4–6/group). E) Echocardiography evaluation of left ventricular fractional shortening in 9-mo-old Mrp4−/− mice compared to age-matched WT (n=4–6/group). F) Real-time PCR analysis of ANF gene expression in the ventricular myocardium of 9-mo-old WT and Mrp4−/− mice (n=4–6). Scale bars = 1 mm (B); 100 μm (C). *P < 0.05; ***P < 0.001.