Figure 5.

TLT shows 1:2 binding stoichiometry with N36. A) CD spectra of solution containing constant concentration of TLT with increasing molecular ratio of N36. Saturation is observed as the molecular ratio of N36 increases. B) θ₂₂₂ changes in solution containing 4 μM TLT with different N36 molecular ratio; an inflection point at TLT:N36 at 1:2.36 indicates that 1 TLT can bind 2 N36. C) Control using the same method shows a 1:1 binding stoichiometry between T1144 and N36. D) N-PAGE shows that 1 TLT can bind 2 N36 to form 6-HB. E) Control N-PAGE shows 1:1 binding stoichiometry between T1144 and N36.