Figure 1.

Gene targeting strategy to produce T4826I-RYR1 mouse. A) cDNA insert with T4826I mutation and SV40 polyA signal (1) and 9.5-kb genomic targeting sequence with KpnI cDNA insertion site (2). B) Targeting construct prior to addition of lox-flanked neo insertion. C) Final targeting cassette with loxP-flanked positive selection cassette and TK negative selection cassette. D) Homologously targeted genomic DNA after excision of neo cassette. See Materials and Methods for details.