Figure 3.

A) Temporal change in core temperature during halothane anesthesia with bed set at 37°C. Animals were induced as described in Materials and Methods and placed on a platform set to 37°C at its surface. Maintenance anesthetic was applied for up to 40 min through a nose cone. Plot shows mean core temperature continuously monitored with WT, HET, and Hom mice (sexes pooled). No WT or Het mice succumbed to MH, whereas 100% of the Hom T4826I animals experienced fulminant MH and died. Arrows indicate mice with the shortest and longest latency to MH. Mice were removed from the moving average of core temperature as they triggered. Inset: representative Hom (top image) and Het (bottom image) mice photographed on the temperature-control bed at the end of the anesthetic procedure (nose cone removed). B, C) Beginning and ending (at the end of 40 min or at the time of MH, whichever came first) core body temperatures, analyzed according to sex and genotype. Female Het T4826I mice (B) displayed reductions in core temperature following halothane exposure (35.7±0.6°C, n=8), whereas T4826I HET males (C) did not (36.5±1.7°C, n=6). Hom T4826I animals (both male and female) displayed marked increases in body temperature following halothane exposure (males, 39.4±0.6°C, n=7; females, 39.9±0.7°C, n=6). *P <0.05; ***P <0.001.