Figure 4.

Temporal change in core temperature during halothane anesthesia with bed set at 41°C. Animals were induced as described in Materials and Methods and placed on a platform set to 41°C at its surface, and maintenance anesthetic was applied for up to 40 min through a nose cone. Plot shows mean core temperature continuously monitored with WT and HET T4826I mice (sexes separated). A, B) Both female (A) and male (B) T4826I HET animals exhibited a gradual rise in core temperature and achieved a significantly higher ending core temperature following halothane anesthesia (Het T4826I 40.8±1.2°C vs. WT 37.7±0.6°C). All Het T4826I males triggered with MH and died, compared to 40% of Het T4826I females. C, D) Beginning and ending (at the end of 40 min or at the time of MH, whichever came first) core body temperatures, analyzed according to sex and genotype. C) Female T4826I HET, but not female WT, had significantly elevated body temperatures at the conclusion of halothane anesthesia compared to their initial temperatures. D) Male Het T4826I and WT animals had significantly elevated body temperature at the conclusion of halothane anesthesia compared to their initial temperature. ***P <0.001.