Figure 2.

Immunization with alum or CpG adjuvants gives rise to a comparable distribution, magnitude, and duration of antigen uptake and presentation in vivo. A–C) Duration and magnitude of antigen persistence were measured as the mean fluorescence intensity (MFI) of EαGFP protein detected at 6, 24, and 72 h postimmunization in B cells (A), CD11c DCs (B), and plasmacytoid DCs (C). D–F) Duration and magnitude of antigen presentation were measured as the MFI of Y-Ae detection of the Eα-MHCII complex at 1, 6, 12, 24, 36, 48, and 72 h postimmunization in B cells (D), CD11c DCs (E), and plasmacytoid DCs (F). Results are means ± sd of 3 animals/group and are representative of ≥3 independent experiments. *P < 0.05 for EαGFP/CpG vs. EαGFP/alum groups; 1-way ANOVA.