Figure 5.

Ear ablation following OVA/alum immunization does not alter the magnitude or phenotype of recall memory responses. A) Mice were immunized with OVA adsorbed to alum, and the injection site was ablated 2 h later. After 2 wk, OVA-specific responses were recalled in control (intact) and ablated mice by immunization with heat-aggregated OVA (HAO). B–E) IgG1 responses were detectable prior to (B) and at 1 wk (C), 2 wk (D) or 3 wk (E) following challenge. Injection site ablation induced a small increase in IgG1 titer at 1 wk postchallenge; no effect of ablation was observed at other time points. F–I) Injection site ablation did not affect the inability of alum/OVA immunized mice to produce IgG2a responses at 1 wk (G), 2 wk (H) or 3 wk (I) postchallenge, compared to prechallenge (F). Results are means ± sd of 5 animals/group. ***P < 0.01.