Fig. 2.

Neurochemical profile of RAGP neurons labeled with the tracer (DiI) injected into right-sided mediastinal nerve sites, where electrical stimulation (MSNS) induced AF. Neurons were immunolabeled for choline acetyltransferase (ChAT; green label), tyrosine hydroxylase (TH; blue label), and DiI (red label). Solid arrow indicates colocalization of ChAT and DiI (16.7% of neurons). Open arrow indicates colocalization of TH and DiI (22% of neurons). Open arrowheads indicate colocalization of DiI with both ChAT and TH (55.6% of neurons). Solid arrowhead indicates a DiI neuron that did not label with either TH or ChAT markers (5.5% of neurons). Scale bar = 40 μm.