Figure 1.

A Petri Dish System with the Upper Segment of Agar Excised to Avoid Direct Contact of Shoot Tissues with the Nutrient Medium.

Arabidopsis seeds were germinated on the surface of vertical agar nursery plates and after 4 to 5 d transferred to treatment plates that had the upper segment of agar removed. By positioning the bases of the hypocotyls in line with the cut edge of the agar, the shoots are able to develop without coming into contact with the agar surface.

[See online article for color version of this figure.]