Figure 6.

Analysis of Suppression of Transgene-Induced Silencing of SD2b Mutants in a Transient Expression System.

(A) GFP fluorescence in leaves of N. benthamiana plants coinfiltrated with 35S-GFP and 35S-SD2b-NLS mutants or 35S-SD2b deletion mutants (as indicated). Coinfiltrations of 35-GFP with vector control (V), 35S-SD2b, or 35S-P19 were used as controls. Photographs were taken under UV light at 4 DPA.

(B) RNA and protein gel blots analysis with samples extracted from infiltrated leaves at 4 DPA. ³²P-labeled GFP and SD2b DNA probes were used. Anti-GFP monoclonal antibody and anti-SD2b polyclonal antibody were used to detect the accumulation of GFP and SD2b proteins in infiltrated leaves, respectively. Methylene blue–stained rRNA and Coomassie blue–stained protein are shown as mRNA and total protein loading controls.