Figure 2.

Analysis of the NDH Complex in Different NDH Mutant Backgrounds.

(A) BN-PAGE analysis of thylakoid protein complexes isolated from wild-type (WT) plants and the indicated mutants. Magnification of the top part of the BN-PAGE gel in Supplemental Figure 1 is shown. After electrophoresis, the gel was stained with Coomassie blue. I, NDH-PSI supercomplex detected in the wild type; II, sub-NDH-PSI supercomplex lacking subcomplex A.

(B) Immunoblot analysis of thylakoid proteins, with the indicated antibodies. Thylakoid proteins were loaded on an equal chlorophyll basis, and the series of dilutions is indicated. An asterisk indicates nonspecific signals.