Figure 3.

Immunodetection of CRR41 and CRR42.

Freshly isolated chloroplasts from various genetic backgrounds were further fractionated into membrane and stromal fractions. Immunoblot analysis was performed with antibodies against CRR41 (A) and HA tag (B). Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (RbcL) and D1 were detected as loading and fractionation controls, respectively. Stromal proteins were loaded by equal protein content, and the series of dilutions is indicated. crr42+CRR42-HA: crr42 transformed with CRR42 cDNA fused to the sequence encoding the HA tag expressed under the control of the CaMV 35S promoter. WT, wild type.