Figure 4.

 Subcellular Localization of a Translational NRT2.4-GFP Fusion Protein in Root Epidermal Cells of the ProNRT2.4:NRT2.4-GFP Transgenic Plant.

(A) and (D) GFP fluorescence (green).

(B) and (E) FM4-64 (red).

(C) and (F) Merged images of GFP fluorescence, FM4-64, and bright field. Yellow color represents the superposition of green and red. e, epidermal cell.

(G) A cross section showing the GFP fluorescence image of ProNRT2.4:NRT2.4-GFP in the lateral root. Transgenic seedlings were grown on full N plates for 7 d and then incubated on MGRL plates without N source for 3 d.

Bars = 20 μm in (A) to (C) and (G) and 10 μm in (D) to (F).