Figure 2.

Defective Lateral Root Development in the arf7 arf19 Double Mutant Causes Leaf Chlorosis under Localized Fe Supply.

(A) and (B) Wild-type (Col-0) and arf7 arf19 seeds were germinated on Fe-free, half-strength MS medium for 7 d. Seedlings were then transferred to segmented agar plates supplied with 75 μM ferrozine and with 50 μM Fe-EDTA in all three segments (homogeneous) or only in the middle segment (localized).

(A) Photographs were taken after 15 d of treatments, and representative plants are shown.

(B) Chlorophyll concentrations of whole shoots of wild-type and arf7 arf19 plants grown under homogeneous or localized supply of 50 μM Fe. Bars represent means ± se; n = 6 plates containing three plants. Asterisk denotes a significant difference according to Student’s t test (P < 0.05). FW, fresh weight; ns, not significant.