Lateral Root Development in Response to Localized Fe Supply in Transgenic Plants with Deregulated Expression of FIT.
Lateral root density (A) and average lateral root length (B) in wild-type (Col-0), Pro35S:FIT, and fit mutant plants as affected by the Fe concentration supplied to the middle segment. Seeds were germinated on Fe-free, half-strength MS medium for 7 d. Then, seedlings were transferred to segmented agar plates supplied with Fe in the middle segment at the indicated concentrations. After 15 d, the number of visible lateral roots (>0.5 mm) and mean lateral root length in the middle segment were determined by image analysis. Bars indicate means ± se, n = 7 to 12 plates with three plants per plate. Different letters indicate significant differences among means (P < 0.05 by Tukey’s test).