Figure 3.

Lateral Root Development in Response to Localized Fe Supply in Transgenic Plants with Deregulated Expression of FIT.

Lateral root density (A) and average lateral root length (B) in wild-type (Col-0), Pro35S:FIT, and fit mutant plants as affected by the Fe concentration supplied to the middle segment. Seeds were germinated on Fe-free, half-strength MS medium for 7 d. Then, seedlings were transferred to segmented agar plates supplied with Fe in the middle segment at the indicated concentrations. After 15 d, the number of visible lateral roots (>0.5 mm) and mean lateral root length in the middle segment were determined by image analysis. Bars indicate means ± se, n = 7 to 12 plates with three plants per plate. Different letters indicate significant differences among means (P < 0.05 by Tukey’s test).