Figure 5.

Effect of Shoot Fe Supply on the Development of Lateral Roots in Wild-Type (Col-0) and irt1 on a Tetrapartite Agar Plate.

(A) Experimental setup for supplying Fe to shoots. Segment a was supplemented with 100 μM citrate or 100 μM Fe(III)-citrate, segment c was supplied with 10 to 600 μM Fe-EDTA, and segments b and d were Fe deficient.

(B) to (E) Chlorophyll concentrations (B), shoot biomass (C), density (D), and average length (E) of lateral roots of wild-type (Col-0) and irt1 plants after growth for 14 d under increasing concentrations of Fe-EDTA in agar segment c. Bars indicate means ± se; n = 6 to 7 plates with three plants each. Different letters indicate significant differences among means (P < 0.05 by Tukey’s test). FW, fresh weight.

[See online article for color version of this figure.]