Figure 3.

Growth of P. syringae in Mature Leaves of the Peroxidase T-DNA and Antisense Knockdown Lines.

As described in Methods, ?0.1 mL of a P. syringae tomato strain DC3000 suspension at OD600 = 0.0005 was infiltrated into mature rosette leaves of 23- to 25-d-old Arabidopsis plants, leaves were harvested at 2, 24, 48, and 72 h after infiltration, and bacterial counts were determined. Data represent the average ± sd. Values for all four lines at 72 h after infiltration were found to be significantly different from each other except Col-0 and prx34:T-DNA with a P value < 0.05 as determined by Student’s t test (A). Values for prx33:T-DNA and prx33:T-DNA;prx34:RNAi at 72 h after infiltration were found to be significantly different from WS, with a P value < 0.05 as determined by Student’s t test (B). At least four biological replicates from independent plants were used for each time point, and the entire experiment was repeated with similar results. prx34, prx34:T-DNA; prx33, prx33:T-DNA; prx33-34-, prx33:T-DNA;prx34:RNAi. cfu, colony-forming units.