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. 2012 Jan 13;24(1):275–287. doi: 10.1105/tpc.111.093039

Figure 5.

Figure 5.

Quantitative RT-PCR Analysis of MAMP-Elicited Genes in Peroxidase-Downregulated Plants.

(A) Basal levels of CYP71A12, CYP79B2, CYP81F2, and MYB51 mRNAs were measured in 23- to 25-d-old plants as described.

(B) Levels of CYP71A12, CYP79B2, CYP81F2, and MYB51 mRNAs were measured at 2 h after infiltration of 0.1 mL of 0.5 μM Flg22 or Elf26. Data represent the average ± sd. All quantitative gene expression measurements were performed using technical and biological triplicates. The experiments were repeated at least two times with similar results. Values for CYP71A12 expression in rbohD and prx33 were found to be significantly different from all other expression values, with a P value < 0.05 as determined by Student’s t test (A). Values for the gene expression levels of each of the four genes in Col-0 and WS, and all genes except CYP79B2 in rbohD, were found to be significantly different from the gene expression levels of all four genes in the peroxidase knockdown lines, with a P value < 0.05 as determined by Student’s t test (B). prx34, prx34:T-DNA; prx33, prx33:T-DNA; prx33-34-, prx33:T-DNA;prx34:RNAi.