Figure 6.

Exogenous H₂O₂ Application Rescues Callose Accumulation in asFBP1.1 and prx34.

Approximately 0.1 mL of 0.5 μM Flg22 was infiltrated into mature rosette leaves of 23- to 25-d-old Arabidopsis plants. At least six independent plants were used as biological replicates, and three rosette leaves were sampled from each plant. The experiment was repeated at least two times. Representative leaves are shown. The infiltrated leaves were detached at 2 h after MAMP treatment and then placed in 12-well sterile plates overnight in 1 mL solution containing either 5 μM, 15 μM H₂O₂, or sterile dH₂O. The leaves were then harvested and stained for callose as described in Methods. Hydrogen peroxide was applied in solution at the time of Flg22 treatment. prx34, prx34:T-DNA.

Bar = 20 μM.