Figure 6. Effects of TORC1 activity on magnetization.

(A)Wild type harboring empty plasmid (Wild type), harboring multi-copy TCO89 plasmid (Multi-TCO89), and tco89Δ harboring empty plasmid (tco89Δ) were grown in selection medium containing 10 mM ferric citrate and 10 nM (red column), 20 nM (green column) rapamycin, or 0.2% dimethyl sulfoxide (DMSO) (blue column) as control. (B) Wild type (blue column) and tco89Δ (red column) are pre-cultured in synthetic complete medium and grown in indicated media containing 10 mM ferric citrate. Indications of media are synthetic complete medium containing 2% or 3% glucose (2% Glu, 3%Glu), 2- or 3-fold concentration of amino acid supplements (2×N, 3×N), or rich medium containing 2%, 3%, or 4% glucose (2% Glu, 3%Glu, and 4%Glu), respectively. Magnetization was measured as in Figure 3B.