Table 3. Relative expression levels of 11 genes in the different biological groups of L. donovani isolates.
| gene | ALL ISOLATES differences between SSG-S and SSG-R | POPULATION A differences between SSG-S and SSG-R | POPULATION B differences between SSG-S and SSG-R |
| CBS | yes (P = 0.050) | yes (P = 0.035) | no (P = 0.565) |
| GCS | yes (P = 0.034) | no (P = 0.061) | no (P = 0.432) |
| MST | yes (P = 0.037) | no (P = 0.719) | yes (P = 0.001) |
| TR | no (P = 0.086) | no (P = 0.063) | no (P = 0.742) |
| ACR2 | no (P = 0.188) | no (P = 0.354) | no (P = 0.251) |
| AQP1 | no (P = 0.095) | no (P = 0.250) | no (P = 0.671) |
| CS | no (P = 0.815) | no (P = 0.801) | no (P = 0.923) |
| ODC | no (P = 0.124) | no (P = 0.487) | no (P = 0.138) |
| TDR1 | no (P = 0.633) | no (P = 0.802) | no (P = 0.829) |
| PRP1 | no (P = 0.847) | no (P = 0.619) | no (P = 0.599) |
| MRPA | no (P = 0.851) | no (P = 0.873) | no (P = 0.399) |
Statistical analysis: P-values comparing the indicated groups of isolates were determined by repeated measures two-way ANOVA with matching by time-points. Data were missing from time-point ‘day 7’ and needed to be excluded from statistical analysis. The complete dataset of average relative gene expression levels (± SD) measured during 8 consecutive days in vitro promastigote growth can be found in dataset S1.