Table 2. Fourteen standard species included in the study and its respective Tm and HRM analysis.
| Tm analysis | HRM analysis | |||||||
| Sp | Tm | SD | 95% CI of Tm | VC | Tm genotype | HRM genotype | %C | %C SD |
| Opossum | 81.02 | 0.09 | 80.97–81.06 | 0.10 | Opossum | Opossum | 81.24 | 15.02 |
| Mouse | 81.63 | 0.12 | 81.57–81.69 | 0.14 | Mouse | Mouse | 94.19 | 4.94 |
| Cow | 81.76 | 0.49 | 81.51–82.00 | 0.60 | Cow | Cow | 83.07 | 16.63 |
| Goat | 81.88 | 0.05 | 81.86–81.91 | 0.06 | Goat | Goat | 88.24 | 13.25 |
| Dog | 82.29 | 0.08 | 82.25–82.33 | 0.09 | Dog | Dog | 90.92 | 13.86 |
| Sheep | 82.29 | 0.03 | 82.26–82.33 | 0.04 | Sheep | Sheep | 98.69 | 1.50 |
| Horse | 82.56 | 0.08 | 82.52–82.60 | 0.10 | Horse | Horse | 93.46 | 9.04 |
| Pig | 82.58 | 0.05 | 82.55–82.60 | 0.06 | Pig | Pig | 95.18 | 9.50 |
| Rabbit | 82.69 | 0.05 | 82.66–82.72 | 0.06 | Rabbit | Rabbit | 98.55 | 1.33 |
| Cat | 83.46 | 0.09 | 83.41–83.50 | 0.11 | Cat | Cat | 78.81 | 22.40 |
| Rat | 83.55 | 0.05 | 83.50–83.59 | 0.05 | Rat | Rat | 89.90 | 6.51 |
| Donkey | 84.39 | 0.04 | 84.36–84.41 | 0.05 | Donkey | Donkey | 87.85 | 22.14 |
| Human | 85.79 | 0.05 | 85.76–85.82 | 0.06 | Human | Human | 96.58 | 2.22 |
| Chicken | 86.27 | 0.05 | 86.24–86.29 | 0.06 | Chicken | Chicken | 93.05 | 18.01 |
SD: standard deviation, CI: confidence interval, VC: variability correlation, %C: confidence percentage, %C SD: standard deviation of confidence percentage, Tm Genotype: Genotype according to Tm from all the replicas.