Table 2. Fourteen standard species included in the study and its respective Tm and HRM analysis.

	Tm analysis					HRM analysis
Sp	Tm	SD	95% CI of Tm	VC	Tm genotype	HRM genotype	%C	%C SD
Opossum	81.02	0.09	80.97–81.06	0.10	Opossum	Opossum	81.24	15.02
Mouse	81.63	0.12	81.57–81.69	0.14	Mouse	Mouse	94.19	4.94
Cow	81.76	0.49	81.51–82.00	0.60	Cow	Cow	83.07	16.63
Goat	81.88	0.05	81.86–81.91	0.06	Goat	Goat	88.24	13.25
Dog	82.29	0.08	82.25–82.33	0.09	Dog	Dog	90.92	13.86
Sheep	82.29	0.03	82.26–82.33	0.04	Sheep	Sheep	98.69	1.50
Horse	82.56	0.08	82.52–82.60	0.10	Horse	Horse	93.46	9.04
Pig	82.58	0.05	82.55–82.60	0.06	Pig	Pig	95.18	9.50
Rabbit	82.69	0.05	82.66–82.72	0.06	Rabbit	Rabbit	98.55	1.33
Cat	83.46	0.09	83.41–83.50	0.11	Cat	Cat	78.81	22.40
Rat	83.55	0.05	83.50–83.59	0.05	Rat	Rat	89.90	6.51
Donkey	84.39	0.04	84.36–84.41	0.05	Donkey	Donkey	87.85	22.14
Human	85.79	0.05	85.76–85.82	0.06	Human	Human	96.58	2.22
Chicken	86.27	0.05	86.24–86.29	0.06	Chicken	Chicken	93.05	18.01

SD: standard deviation, CI: confidence interval, VC: variability correlation, %C: confidence percentage, %C SD: standard deviation of confidence percentage, Tm Genotype: Genotype according to Tm from all the replicas.