Table 4. Amplification results from field samples (Table S1) showing one or two peaks in the melting curve.
| Lowest temperature peak analysis | Highest temperature peak analysis | |||||||||||
| Sample | Tm1 | SD | Tm1 genotype | HRM genotype | HRM %C | SD | Tm2 | SD | Tm2 genotype | HRM genotype | HRM %C | SD |
| 1 | 86.07 | 0.07 | Human | Human | 83.29 | 14.60 | - | - | - | - | - | - |
| 2 | 85.96 | 0.06 | Human | Human | 90.98 | 5.02 | - | - | - | - | - | - |
| 3 | 85.96 | 0.05 | Human | Human | 81.84 | 6.48 | - | - | - | - | - | - |
| 4 | 86.15 | 0.06 | Human | Human | 75.41 | 2.02 | - | - | - | - | - | - |
| 5 | 86.03 | 0.04 | Human | Human | 90.91 | 3.56 | - | - | - | - | - | - |
| 6 | 82.04 | 0.08 | Dog | Dog | 56.48 | 3.30 | - | - | - | - | - | - |
| 7 | 82.02 | 0.15 | Dog | Dog | 53.68 | 25.81 | 86.14 | 0.04 | Human | Human | 64.56 | 14.75 |
| 9 | 84.74 | 0.02 | ND | ND | ND | ND | 86.13 | 0.03 | Human | Human | 63.11 | 9.50 |
| 10 | 84.87 | 0.21 | ND | ND | ND | ND | 86.10 | 0.04 | Human | Human | 78.90 | 15.17 |
| 12 | 81.16 | 0.01 | Opossum | Opossum | 86.02 | 11.19 | 86.10 | 0.03 | Human | Human | 82.24 | 3.30 |
| 14 | 86.15 | 0.02 | Human | Human | 56.35 | 6.93 | - | - | - | - | - | - |
| 15 | 84.85 | 0.09 | ND | ND | ND | ND | 86.20 | 0.06 | Human | Human | 40.76 | 11.44 |
| 16 | 86.19 | 0.06 | Human | Human | 57.23 | 11.38 | - | - | - | - | - | - |
| 19 | 84.85 | 0.08 | ND | ND | ND | ND | 86.16 | 0.01 | Human | ND | ND | ND |
| 20 | 86.58 | 0.02 | Chicken | Chicken | 88.71 | 7.08 | - | - | - | - | - | - |
Tm1: melting temperature of peak 1, Tm2: melting temperature of peak 2, SD: standard deviation, HRM %C: confidence percentage of HRM analysis, ND: not determined or not recognized, -: No secondary peak.