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. 2012 Feb 28;6(2):e1536. doi: 10.1371/journal.pntd.0001536

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Moi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Sixty serum samples exhibiting neutralizing activity to DENV were analyzed for presence of infection-enhancement activity to each of the four DENV serotypes: (A) DENV-1, (B) DENV-2, (C) DENV-3 and, (D) DENV-4. Infection-enhancement activity to each DENV serotype was determined by using FcγR-expressing cells by the formula: (mean plaque count at 1∶10 serum dilution)/(mean plaque count in the absence of human serum samples), and expressed as fold enhancement to each DENV serotype. Closed bars indicate serum samples with neutralizing titer PRNT₅₀≥10 to the indicated DENV serotype and open bars indicate serum samples with neutralizing titer PRNT₅₀<10 as determined using FcγR-negative BHK cells. (*) indicates P<0.05.