Figure 3. Thermal transition of PTPρ wild-type and mutants.

(A) PTPρ wild-type, N1128I, Q987K and D927G were heated from 10°C to 72°C in a 0.1-cm quartz cuvette at 0.2 mg/ml in 20 mM Tris/HCl, pH 7.5 containing 0.2 M NaCl and 0.4 mM DTT. The dichroic activity at 209 nm was monitored continuously every 0.5°C. The inset shows the first derivative of the same data. (B) PMTV data recorded in the same experiments shown in (A).