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. 2012 Feb 28;7(2):e32741. doi: 10.1371/journal.pone.0032741

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Hurt et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Western blot of crude cell lysates (Lys) and supernatants (Sup) from P. pastoris X33 untransformed controls and from P. pastoris X33 expressing r-hPAP. Blot probed with anti-hPAP antiserum. (B) DiFMUP fluorometric enzyme assay with concentrated supernatants (secreted fractions) in comparison to native hPAP from human semen. 0.625 µg total protein used per reaction. Data are plotted as an average of duplicate trials ± standard deviation (SD).