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. 2012 Feb 28;7(2):e32741. doi: 10.1371/journal.pone.0032741

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Hurt et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Western blot of crude cell lysates from untransformed X33 cells and transformants expressing hPAP with (r-hPAP) or without (-SP) a signal peptide. Blot probed with anti-hPAP antiserum. Equivalent amounts of total protein were loaded in each lane. (B) DiFMUP fluorometric enzyme assay with the indicated crude cell lysates. Data are plotted as an average of duplicate trials ± SD.