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. Author manuscript; available in PMC: 2013 Feb 28.
Published in final edited form as: Biochemistry. 2012 Feb 10;51(8):1730–1739. doi: 10.1021/bi201159b

Figure 10.

Figure 10

Covalent linkage of the head group to the acridine moiety is necessary for the high potency of m-AMSA as a topoisomerase II poison. The ability of the detached head group (HG, closed circles) or a 1:1 mixture of head group + acridine (HG + ACR, open circles) to stimulate topoisomerase IIα-mediated DNA cleavage is shown. A control experiment assessing the effects of acridine alone on the DNA cleavage activity of topoisomerase IIα (ACR, squares) also is shown. Error bars represent the standard deviation of three independent experiments.