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. Author manuscript; available in PMC: 2013 Feb 28.
Published in final edited form as: Biochemistry. 2012 Feb 10;51(8):1730–1739. doi: 10.1021/bi201159b

Figure 11.

Figure 11

Partial redox-dependence of the isolated m-AMSA head group as a topoisomerase II poison. The effects of 25 µM m-AMSA or 3 mM m-AMSA head group on the cleavage of negatively supercoiled plasmid DNA by human topoisomerase IIα in the absence (closed bars) or presence (open bars) of 3 mM dithiothreitol (DTT) are shown. A control experiment carried out in the absence of drugs also is shown (No Drug). DNA cleavage levels are relative to those induced by the enzyme in the absence of drug or DTT. Error bars represent the standard deviation of three independent experiments.