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. Author manuscript; available in PMC: 2013 Feb 28.
Published in final edited form as: Biochemistry. 2012 Feb 10;51(8):1730–1739. doi: 10.1021/bi201159b

Figure 4.

Figure 4

DNA cleavage site specificity and utilization by human topoisomerase IIα in the presence of m-AMSA and derivatives. A singly end-labeled linear 4332 bp fragment of pBR322 was used as the cleavage substrate. An autoradiogram of a polyacrylamide gel is shown. DNA cleavage reactions were carried out in the absence of drug (TIIα), or in the presence of 10 µM m-AMSA; 25 µM AMSA, 1'-OH 3’-OCH3, or 1’-OH; or 100 µM o-AMSA or 1'-OH 2’-OCH3. DNA standards (DNA) also are shown. Results are representative of three independent experiments.