Table 1.
DNA Intercalation by m-AMSA and Derivativesa
| Compound | Fully Relaxed | Fully Supercoiled |
|---|---|---|
| Concentration (µM) | Concentration (µM) | |
| m-AMSA | 25 | 150 |
| AMSA | 5–10 | 50 |
| o-AMSA | 10 | 75 |
| 1’-OH 3’-OCH3 | 15 | 150 |
| 1’-OH | 5 | 25 |
| 1’-OH 2’-OCH3 | 5 | 25–50 |
| 3’-OCH3 | 50–75 | >150 |
| N-Phenyl | 15–25 | 75 |
| 2’-OCH3 | 15 | 150 |
| 9-aminoacridine | 5 | 50 |
| 4-methyl-m-AMSA | 15 | 75 |
| Acridine | 75 | ≫150 |
| Ethidium Bromide | 1 | 5–7.5 |
a
The concentrations of compounds required to convert the plasmid substrate to a “fully relaxed” or “fully supercoiled” population was assessed by the topoisomerase I DNA supercoiling assay described in Figure 5.