Fig. 3.

The influence of Id1 over-expression and silencing on the activation of the PI3K/Akt/NFκB/survivin signalling pathway and proliferation of EPCs. a, b Left proteins(Id1, p-PI3K, p-Akt, p-IκB, survivin, and nuclear NFκB/p65) from EPCs transfected with Ad-Id1 or si-Id1 were collected and subjected to western blot analysis. Right densitometric analysis of Id1, p-PI3K, p-Akt, p-IκB, NFκB/p65 (nuclear), and survivin protein expression levels were determined by Quantity One. c, d The proliferation of EPCs was examined by MTS assays. The proliferation of EPCs transfected with Ad-Id1 was enhanced to about 200% as compared with that of Ad-GFP-transfected EPCs. Whereas knockdown of endogenous Id1 significantly reduced the proliferation of EPCs compared with the control group. The results are expressed as the mean ± SD. ^# P < 0.05 versus control