Figure 1.
Sequence profiles of the mtDNA mutations in LA9 and LMTK− cells. A–F show six heteroplasmic mtDNA polypeptide mutations. These include ND2 C4794T (A), COI T6589C (B), ND5 T12048C (C), ND6 13885insC (D), [tRNAArg 9821(9A)] versus [tRNAArg 9821(10A)] (E), and ND5 13053insC (F) found in LA9W1998 and/or LA9W2002. In E, the tRNAArg 9821 locus 9As is a wild-type allele and 10As is a mutant. This locus is heteroplasmic in LA9W1998, homoplasmic wild type in LA9W2002 (top; 9As), and homoplasmic mutant in LMTK− (bottom; 10As). With the exception of tRNAArg 9821, the top trace is mutant, and the bottom is wild type. The wild-type genotype for C3H and 129 mice is [ND2 4794C]-[COI 6589T]-[tRNAArg 9821(9A)]-[ND5 12048T]-[ND5 13050(7C)]-[ND6 13885insC].