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. Author manuscript; available in PMC: 2013 Mar 9.
Published in final edited form as: J Mol Biol. 2012 Jan 27;416(5):678–696. doi: 10.1016/j.jmb.2012.01.015

Figure 9. In vivo formaldehyde cross-linkage of Tp0956 and Tp0957.

Figure 9

Samples were resolved in two identical gels and then electrotransferred to two separate membranes. The membranes were individually probed with either an anti-Tp0956 (α-Tp0956; left panel) or an anti-Tp0957 (α-Tp0957; right panel) antibody and visualized by an enhanced chemiluminescent detection using an anti-goat IgG-HRP. Lane 1 shows T. pallidum not exposed to the formaldehyde; lane 2, formaldehyde cross-linked treponemes, and lane 3, boiled treponemes to break the cross-links prior to SDS PAGE. The yellow arrows represent the putative positions of the cross-linked hetero-hexameric complex, while the red and green arrows denote the positions of monomeric Tp0956 and Tp0957, respectively. The positions of pre-stained molecular-weight standards (M; values are in kDa) are indicated in the image of the blot.