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Double BrdU-NeuN immunohistochemistry on a sagittal section from a neurosphere-grafted bridged nerve at 90 days after lesion. One given field was sequentially photographed with fluorescent wavelengths revealing, respectively, Alexa-594-labeled BrdU immunoreactivity (a) and Alexa-488-NeuN one (b). Note the extensive nuclear colocalization of the mature neuron marker NeuN with BrdU (b). Scale bars 100 mm.
