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OMV production from P. aeruginosa under the ciprofloxacin-induced SOS response. (a) Growth of the wild-type (PAO1) and the LexA noncleavable strains. Both strain were grown in LB with shaking for 2 hrs, and then ciprofloxacin (CPX) was added. The culture continued for 6 hrs. OMVs were isolated from the wild-type and the lexAN cells treated with or without ciprofloxacin (CPX). (b) Quantification of OMV proteins by Bradford (n = 7) and (c) OMV lipids by weight (n = 3). (*P < 0.05).
