Fig. 1.

Construction of recombinant MVA-survivin. a BHK cells were infected with MVA and transfected with mouse survivin transfer vector. The mouse survivin expression cassette was inserted into the wildtype IGR3 site by homologous recombination. b DNA was extracted from ultrapurified isolate C7 and then used in PCR to detect for the presence of wildtype MVA. Isolates from uninfected and wtMVA-infected BHK cells were used as negative and positive controls, respectively. The mouse survivin expression cassette and wildtype IGR3 site have lengths of 1.6 and 300 bp, respectively. c Ultrapurified C7 lysate was tested for expression of survivin by Western blot analysis. Survivin is a protein of ~17 kDa. d Pan02 tumor cell line and normal pancreas were tested for expression of survivin by Western blot analysis