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. Author manuscript; available in PMC: 2012 Feb 29.
Published in final edited form as: Mol Cell. 2008 Jul 25;31(2):278–286. doi: 10.1016/j.molcel.2008.05.019

Fig. 5. Growth arrest of S. cerevisiae by zymocin is relieved by tRNA repair.

Fig. 5

(A) Serial 10-fold dilutions of the indicated S. cerevisiae strains were spotted on YPD agar plates (control) or YPD plates containing 45% (v/v) zymocin. (B) RT-PCR analysis of tRNA levels in yeast cells grown in the presence or absence of zymocin. The PCR products were analyzed by agarose gel electrophoresis and stained with ethidium bromide. In strains expressing split domains of tRNA ligase, the source of the sealing domain (ligase) is indicated above the + sign and the source of the healing domain (kinase-CPD) is specified below.