. Author manuscript; available in PMC: 2012 Jul 1.

Published in final edited form as: Anal Biochem. 2011 Mar 9;414(1):38–46. doi: 10.1016/j.ab.2011.02.009

Table 5.

Average percentage of recovery from best surface and improvement by addition of 1% BSA for the 8 peptides tested

Peptide	Surface	Recovery (%)
Peptide	Surface	Without BSA	Addition of 1% BSA
Nesfatin-1	borosilicate glass	32.0 ± 1.5	90.7 ± 3.4
PYY	borosilicate glass	67.7 ± 6.8	96.0 ± 2.7
Leptin	borosilicate glass	60.3 ± 1.7	92.7 ± 3.3
CRF	borosilicate glass	61.5 ± 1.6	90.6 ± 2.9
GLP-1	borosilicate glass	39.6 ± 1.2	85.3 ± 2.8
Insulin	borosilicate glass	54.6 ± 2.1	95.7 ± 2.4
Ghrelin	polypropylene plastic	90.3 ± 1.9	93.9 ± 2.5
CCK-8S	polypropylene plastic	86.3 ± 1.2	98.3 ± 0.8

Borosilicate glass tubes gave maximal recovery for nesfatin-1, PYY, leptin, GLP-1 and CRF while polypropylene plastic was best for ghrelin and CCK-8S. Note: Polyallomer plastic, that is mostly used as material for high speed centrifugation tubes, gave a better recovery of ¹²⁵I-insulin (68.3 ± 5.8%) than borosilicate glass. Addition of 1% BSA significantly improved recovery.