Fig. 2.

RT-PCR product for Panx2 (140 bp) was found in freshly dissociated endothelial cells (ECs; a, left panel) and RT-PCR products for Panx1 (125 bp) and Panx2 (140 bp) were found in freshly dissociated smooth muscle cells (SMCs; b, left panel) from rat MCA. The right panels show RT-PCR products indicating the absence of SM22α (325 bp) and the presence of endothelial nitric oxide synthase (eNOS; 356 bp) in dissociated endothelial cells (a, right panel) and vice versa in dissociated smooth muscle cells (b, right panel). No product was detected if reverse transcriptase was omitted (data not shown). Data are representative of 3 separate preparations.